internal control glyceraldehde 3 phosphate dehydrogenase  (Cell Signaling Technology Inc)

Journal: Frontiers in Pharmacology

Article Title: Chinese classical decoction Wuwei Xiaodu Drink alleviates gout arthritis by suppressing NLRP3-Mediated inflammation

doi: 10.3389/fphar.2024.1388753

Figure Lengend Snippet: Primers used for reverse transcription-quantitative polymerase chain reaction.

Article Snippet: To block non-specific binding sites on the membranes, they were incubated with skim milk diluted to a concentration of 5% for 1 h. Following this step, primary antibodies including NLRP3 (1:1000), IL-1β (1:1000), Caspase1 (1:1000), IL-18 (1:1000) and internal control GAPDH (1:1000) from Abcam (Cambridge MA., United States) were added to the membranes and allowed to incubate overnight at a temperature of four degrees Celsius.

Techniques: Sequencing

Journal: Immunity, Inflammation and Disease

Article Title: Dendritic cells infected with recombinant adenoviral vector encoding mouse fibroblast activation protein‐α and human livin α exert an antitumor effect against Lewis lung carcinoma in mice

doi: 10.1002/iid3.1011

Figure Lengend Snippet: Identification of LLC tumor‐isolated CAFs and determination on expressions of FAP and livin α in LLC. (A–C) Western blot was performed to measure protein expressions of CD45, CD31, α‐SMA, and FAP in CAFs isolated from LLC‐bearing mice as well as the protein expression of livin α in mouse LLC tumors. GAPDH was used as the loading control. +++ p < .001, versus lysate; ^^^ p < .001, versus tumor; ## p < .001, versus Para. CAFs, cancer‐associated fibroblasts; LLC, Lewis lung carcinoma; Para, paracancerous tissues.

Article Snippet: Following incubation with blocking buffer (P0231, Beyotime), Western blot was performed by incubating the membranes with primary antibodies against FAP (NB110‐85534, 88 kDa, Novus Biologicals), livin α (NB100‐56145, 33 kDa, Novus Biologicals), CD45 (ab10558, 147 kDa, Abcam), CD31 (ab281583, 82 kDa, Abcam), α‐SMA (ab5694, 42 kDa, Abcam), and internal control GAPDH (ab8245, 37 kDa, Abcam) at 4°C overnight.

Techniques: Isolation, Western Blot, Expressing

Journal: Immunity, Inflammation and Disease

Article Title: Dendritic cells infected with recombinant adenoviral vector encoding mouse fibroblast activation protein‐α and human livin α exert an antitumor effect against Lewis lung carcinoma in mice

doi: 10.1002/iid3.1011

Figure Lengend Snippet: Identification of rAd‐infected DCs in vitro. (A–C) rAd‐FAP, rAd‐hlivin α, and rAd‐FAP/hlivin α were constructed using mouse FAP cDNA and human livin α cDNA, and then transduced into mouse bone marrow‐derived DCs, respectively. For evaluation of transduction efficacy, western blot was performed to measure protein expressions of FAP and livin α in cells, with GAPDH functioned as a loading control. *** p < .001, versus 1#; +++ p < .001, versus 2#; ^^^ p < .001, versus 3#. DCs, dendritic cells; FAP, fibroblast activation protein‐α; rAd‐FAP, recombinant adenoviral vector encoding mouse FAP cDNA; rAd‐hlivin α, recombinant adenoviral vector encoding human livin α cDNA.

Article Snippet: Following incubation with blocking buffer (P0231, Beyotime), Western blot was performed by incubating the membranes with primary antibodies against FAP (NB110‐85534, 88 kDa, Novus Biologicals), livin α (NB100‐56145, 33 kDa, Novus Biologicals), CD45 (ab10558, 147 kDa, Abcam), CD31 (ab281583, 82 kDa, Abcam), α‐SMA (ab5694, 42 kDa, Abcam), and internal control GAPDH (ab8245, 37 kDa, Abcam) at 4°C overnight.

Techniques: Infection, In Vitro, Construct, Derivative Assay, Transduction, Western Blot, Activation Assay, Recombinant, Plasmid Preparation